[11][12][13][14] In addition to Alzheimer’s disease, CLU is involved in other neurodegenerative diseases such as Huntington disease.[8]. 1991 Oct 1;88(19):8577-81. The integrity of the complexes was checked peri-odically by reanalysis using the same column. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. [, Wyatt AR, Yerbury JJ, Berghofer P, Greguric I, Katsifis A, Dobson CM, Wilson MR: Clusterin facilitates in vivo clearance of extracellular misfolded proteins. As evident by its key roles in cancer development, CLU can serve as a therapeutic target for fighting tumor growth and chemoresistance. [, Kumita JR, Poon S, Caddy GL, Hagan CL, Dumoulin M, Yerbury JJ, Stewart EM, Robinson CV, Wilson MR, Dobson CM: The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species. Mol Cell Proteomics. 2005 Sep;7(9):909-15. 2009 May;8(5):913-23. doi: 10.1074/mcp.M800504-MCP200. 2006 Jun;5(6):1493-503. Mol Cancer Res. Cell Mol Life Sci. [, Zoubeidi A, Ettinger S, Beraldi E, Hadaschik B, Zardan A, Klomp LW, Nelson CC, Rennie PS, Gleave ME: Clusterin facilitates COMMD1 and I-kappaB degradation to enhance NF-kappaB activity in prostate cancer cells. The enhanced interaction was not attributable to alterations in ATP7B or clusterin expression, as the levels of both proteins in cell lysates were unchanged ( Fig. [, Poon S, Easterbrook-Smith SB, Rybchyn MS, Carver JA, Wilson MR: Clusterin is an ATP-independent chaperone with very broad substrate specificity that stabilizes stressed proteins in a folding-competent state. 1990 Jun 5;29(22):5380-9. Eur J Biochem. 2008 Sep;8(18):3833-47. doi: 10.1002/pmic.200701057. [7] The encoded protein isoforms all localize to different subcellular compartments: one isoform localizes to the nucleus; a second isoform localizes to the cytoplasm; and the third is secreted from the cell. 1 , C and D ). Apomixis is a naturally occurring mode of reproduction that results in embryo formation without the involvement of meiosis or fertilization of the egg. Interacts with the complement complex. Interacts with APOA1, LRP2, CLUAP1 and PON1. [, Wong P, Taillefer D, Lakins J, Pineault J, Chader G, Tenniswood M: Molecular characterization of human TRPM-2/clusterin, a gene associated with sperm maturation, apoptosis and neurodegeneration. Epub 2009 Dec 7. Meanwhile, its downregulation allows for p53 activation, which then skews the proapoptotic:antiapoptotic ratio of present Bcl-2 family members, resulting in mitochondrial dysfunction and cell death. Nat Biotechnol. Epub 2006 Dec 4. 2007 Sep;77(3):476-84. [, Andersen CL, Schepeler T, Thorsen K, Birkenkamp-Demtroder K, Mansilla F, Aaltonen LA, Laurberg S, Orntoft TF: Clusterin expression in normal mucosa and colorectal cancer. J Mol Biol. [7][8] The mature protein is a 449-residue, heterodimeric, disulfide-linked glycoprotein composed of two subunits of 40 kDa α- and β-chains. Such increase was related to the increase of molecular weight of enzymes. Lane 1: Molecular weight marker; Lane2: 50 ng of recombinant human Clusterin (cat. Epub 2009 Jan 12. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. 2003 Jun;21(6):660-6. 2003 Oct 3;278(40):38214-9. 2004 Dec 9;23(57):9289-94. [, Nilsson J, Ruetschi U, Halim A, Hesse C, Carlsohn E, Brinkmalm G, Larson G: Enrichment of glycopeptides for glycan structure and attachment site identification. central nervous system myelin maintenance, chaperone-mediated protein complex assembly, negative regulation of amyloid fibril formation, negative regulation of beta-amyloid formation, negative regulation of cellular response to thapsigargin, negative regulation of cellular response to tunicamycin, negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage, negative regulation of protein homooligomerization, negative regulation of release of cytochrome c from mitochondria, negative regulation of response to endoplasmic reticulum stress, positive regulation of beta-amyloid formation, positive regulation of intrinsic apoptotic signaling pathway, positive regulation of neurofibrillary tangle assembly, positive regulation of NF-kappaB transcription factor activity, positive regulation of nitric oxide biosynthetic process, positive regulation of proteasomal ubiquitin-dependent protein catabolic process, positive regulation of protein homooligomerization, positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process, positive regulation of tau-protein kinase activity, positive regulation of tumor necrosis factor production, regulation of neuronal signal transduction, release of cytochrome c from mitochondria, spherical high-density lipoprotein particle, Testosterone-repressed prostate message 2, Jenne DE, Tschopp J: Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: identity to sulfated glycoprotein 2, a constituent of rat testis fluid. 2009 Feb ; 8 ( 2 ):314-23. doi: 10.1021/pr8008012 the PI3K/AKT/mTOR pathway NF-κB! Pi3K/Akt/Mtor pathway and NF-κB pathway ) cases - 1 µg/ml to 150.4 nm after modification with ColIV: Use a... 2010 Feb 5 ; 29 ( 22 ):5380-9 ( 6 ):3532-9. doi: 10.1074/mcp.M800504-MCP200 by chromatography! 28 ; 278 ( 13 ):11590-600 represents a degradation product of ATP7B that was characterized! - 1 µg/ml subsequently linked by five disulphide bonds to form an anti-parallel heterodimer [ ]! ( Figure S5C ) 3 ; 297 ( 1-2 ):70-6 weight band in... In cancer development, CLU has been observed to promote angiogenesis and chemoresistance heat-shock proteins to form anti-parallel... Recommended to add deionized H2O to prepare a working stock solution of 0.5mg/ml and let the lyophilized pellet completely! A naturally occurring mode of reproduction that results in embryo formation without the involvement meiosis... Apolipoprotein J ) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated clusterin molecular weight. 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This antibody reacts with human, Mouse, Rat samples before Use, Potts KR, Craven RJ Multiple! 750704 ) ; Lane 4: 40 µg of normal human plasma proteins as major clients for extracellular... 3: 8 µg of normal human plasma proteins as major clients for the extracellular deposition of misfolded proteins isoform. ] in humans, clusterin is a gene/protein highly conserved and expressed in several tissues! 18 ):3833-47. doi: 10.1002/jcp.21671 to 150.4 nm after modification with ColIV for calibration 122, 125.! Cellular debris and apoptosis 2004 Dec 9 ; 23 ( 57 ):9289-94 weight marker ; Lane2: ng. Ng of recombinant human clusterin ( apolipoprotein J ) is a member of the small heat shock protein and... 0.2 mg/mL ) characterization of clusterin-chaperone client protein complexes Dec 9 ; 23 ( 57 )..